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基于凹面光栅的特定蛋白分析仪结构设计及性能分析

Structural Design and Performance Analysis of Specific Protein Analyzer Based on Concave Grating

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摘要

为解决罗兰光栅光谱成像不?#23383;?#25509;读取、平面场凹面光栅成像算法复杂等问题,设计了一种基于全息凹面光栅原理的用于特定蛋白分析仪生化检测的分光光度计。该结构?#26376;?#20848;光栅为基底,采?#30431;?#36879;镜作为过渡元件,将罗兰光栅的曲面成像过渡成平场光谱,同时采用光学设计软件Zemax对该结构进行模拟和优化。优化结果表明,分光光度计能够实现光谱平场化,工作波段为320~800 nm,最高分辨率可达0.5 nm,整体分辨率优于1.7 nm,可被光电二极管阵列直接读取,结构特性满足特定蛋白分析仪的测量要求。样机实验结果与模拟结果一致,仪器重复性、准确?#21462;?#31283;定性及线性度均达到行业标准。

Abstract

In order to solve such problems as difficulty in directly reading the spectral imaging of Rowland grating and the complex imaging algorithm for flat-field concave gratings, we design a spectrophotometer based on principle of the holographic concave grating for biochemical detection of specific protein analyzers. This structure is based on the Rowland grating, and uses two lenses as the transition elements to transform the curved surface imaging of Rowland grating to the flat-field spectrum. Meanwhile, we simulate and optimize the structure with the optical design software Zemax. The optimization results show that the spectrophotometer whose working band is 320-800 nm with the highest resolution of 0.5 nm and the overall resolution of 1.7 nm, can be directly read by the photodiode array and can achieve plane spectrum. The structural characteristics meet the measurement requirements of specific protein analyzers. The experimental results of the prototype are consistent with the simulation results, and the repeatability, accuracy, stability, and linearity of the instrument are all up to the industry standards.

Newport宣传-MKS新实验室计划
补充资料

DOI:10.3788/AOS201939.0830002

所属栏目:光谱学

基金项目?#33322;?#33487;省自然科学基金青年基金(BK20160190);

收稿日期:2019-03-13

修改稿日期:2019-04-23

网络出版日期:2019-08-01

作者单位    点击查看

李俊纬?#33322;?#21335;大学物联网工程学院, 江苏 无锡 214122
倪屹?#33322;?#21335;大学物联网工程学院, 江苏 无锡 214122
郭瑜?#33322;?#21335;大学物联网工程学院, 江苏 无锡 214122
张啸天?#33322;?#21335;大学物联网工程学院, 江苏 无锡 214122
钟道鸿?#33322;?#21335;大学物联网工程学院, 江苏 无锡 214122
樊俊星?#20309;?#38177;克劳斯麦迪医疗仪器有限公司, 江苏 无锡 214028

联系人作者:倪屹([email protected])

备注?#33322;?#33487;省自然科学基金青年基金(BK20160190);

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引用该论文

Junwei Li, Yi Ni, Yu Guo, Xiaotian Zhang, Daohong Zhong, Junxing Fan. Structural Design and Performance Analysis of Specific Protein Analyzer Based on Concave Grating[J]. Acta Optica Sinica, 2019, 39(8): 0830002

李俊纬, 倪屹, 郭瑜, 张啸天, 钟道鸿, 樊俊星. 基于凹面光栅的特定蛋白分析仪结构设计及性能分析[J]. 光学学报, 2019, 39(8): 0830002

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